7WZS
Crystal structure of Chromobacterium violaceum effector CopC in complex with host calmodulin and caspase-7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2021-01-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 90.871, 90.871, 426.106 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.650 - 3.600 |
R-factor | 0.2779 |
Rwork | 0.274 |
R-free | 0.31370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k88 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.352 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.650 | 3.690 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.066 | 1.120 |
Number of reflections | 21855 | 1562 |
<I/σ(I)> | 18.5 | 2.1 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 8.3 | 8 |
CC(1/2) | 0.999 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 100 mM Bis-Tris propane pH 7.5, 1.5 M lithium sulfate |