7WSL

PD-1 in complex with Dostarlimab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97949
Spacegroup name	P 1
Unit cell lengths	39.600, 44.870, 76.960
Unit cell angles	93.80, 98.18, 97.95

Refinement procedure

Resolution	28.543 - 1.534
R-factor	0.1615
Rwork	0.161
R-free	0.17830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ggs
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.543	1.590
High resolution limit [Å]	1.530	1.530
Number of reflections	73835	7304
<I/σ(I)>	14.5
Completeness [%]	95.3
Redundancy	3.7
CC(1/2)	0.997	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M trimethylamine N-oxide dihydrate, 0.1M Tris pH 8.5, 20% w/v PEG 3350