7WKX

IL-17A in complex with the humanized antibody HB0017

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03923
Spacegroup name	C 1 2 1
Unit cell lengths	301.142, 50.829, 117.216
Unit cell angles	90.00, 101.27, 90.00

Refinement procedure

Resolution	45.200 - 2.810
R-factor	0.2078
Rwork	0.205
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vxs 5drw
RMSD bond length	0.009
RMSD bond angle	1.772
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.067	0.067
Number of reflections	41319
<I/σ(I)>	15.5	2.2
Completeness [%]	96.5
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	4% v/v Tacsimate pH 7.0, 12% w/v Polyethylene glycol 3,350