7WJV
Crystal structure of human liver FBPase complexed with an covalent inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.641, 83.617, 278.717 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.453 - 1.724 |
R-factor | 0.1746 |
Rwork | 0.174 |
R-free | 0.19840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zwk |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.453 | 1.750 |
High resolution limit [Å] | 1.720 | 1.720 |
Number of reflections | 167125 | 7923 |
<I/σ(I)> | 8.5 | |
Completeness [%] | 99.8 | |
Redundancy | 13.2 | |
CC(1/2) | 0.992 | 0.770 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 291 | 0.1 M Tris (pH 6.8), 14% (v/v) EtOH, 7.5mM AMP |