7WH1
structure of C elegans BCMO-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 148.756, 148.756, 136.294 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.488 - 1.900 |
R-factor | 0.1598 |
Rwork | 0.158 |
R-free | 0.18640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rse |
RMSD bond length | 0.007 |
RMSD bond angle | 0.843 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.093 | |
Rpim | 0.018 | 0.409 |
Number of reflections | 58459 | 4402 |
<I/σ(I)> | 41.7 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 26.4 | |
CC(1/2) | 0.999 | 0.733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 289 | ammonium acetate, ammonium sulfate |