7WH1

structure of C elegans BCMO-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	I 41 2 2
Unit cell lengths	148.756, 148.756, 136.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.488 - 1.900
R-factor	0.1598
Rwork	0.158
R-free	0.18640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rse
RMSD bond length	0.007
RMSD bond angle	0.843
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.093
Rpim	0.018	0.409
Number of reflections	58459	4402
<I/σ(I)>	41.7	2.3
Completeness [%]	100.0	100
Redundancy	26.4
CC(1/2)	0.999	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	289	ammonium acetate, ammonium sulfate