7WH1

structure of C elegans BCMO-2

Summary for 7WH1

• Entry DOI: 10.2210/pdb7wh1/pdb
• Descriptor: Beta-Carotene 15,15'-MonoOxygenase, FE (III) ION, GLYCEROL, ... (6 entities in total)
• Functional Keywords: nonheme iron, dioxygenase, caenorhabditis elegans, retinoid, beta-carotene, metal binding protein
• Biological source: Caenorhabditis elegans
• Total number of polymer chains: 1
• Total formula weight: 63735.75

Authors

Pan, W.,Liu, L. (deposition date: 2021-12-29, release date: 2022-02-02, Last modification date: 2023-11-29)

Primary citation

Pan, W.,Zhou, Y.L.,Wang, J.,Dai, H.E.,Wang, X.,Liu, L.
Structural and Functional Analysis of Nonheme Iron Enzymes BCMO-1 and BCMO-2 from Caenorhabditis elegans .
Front Mol Biosci, 9:844453-844453, 2022

PubMed Abstract: Carotenoid metabolism is critical for diverse physiological processes. The nematode has two genes that are annotated as β-carotene 15,15'-monooxygenase (BCMO) and are 17 centimorgan apart on chromosome II, but the function of BCMO-1 and BCMO-2 remains uncharacterized. Sequence homology indicates that the two enzymes belong to the carotenoid cleavage dioxygenase family that share a seven-bladed β-propeller fold with a nonheme iron center. Here we determined crystal structures of BCMO-1 and BCMO-2 at resolutions of 1.8 and 1.9 Å, respectively. Structural analysis reveals that BCMO-1 and BCMO-2 are strikingly similar to each other. We also characterized their β-carotene cleavage activity, but the results suggest that they may not act as β-carotene 15,15'-oxygenases.

PubMed: 35223999
DOI: 10.3389/fmolb.2022.844453

Experimental method

X-RAY DIFFRACTION (1.9 Å)