7W5P
Crystal Structure of the dioxygenase CcTet from Coprinopsis cinereain bound to 12bp N6-methyldeoxyadenine (6mA) containing duplex DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.788, 105.198, 196.717 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.180 - 2.300 |
R-factor | 0.2292 |
Rwork | 0.228 |
R-free | 0.25730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vpn |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.111 | 0.084 | 0.726 |
Rmeas | 0.116 | 0.088 | 0.753 |
Rpim | 0.032 | 0.026 | 0.198 |
Total number of observations | 1309069 | ||
Number of reflections | 96239 | 5098 | 4767 |
<I/σ(I)> | 4.4 | ||
Completeness [%] | 99.5 | 98.6 | 99.8 |
Redundancy | 13.6 | 12.2 | 14.1 |
CC(1/2) | 0.955 | 0.863 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M MES, pH 6.5, 20% PEG 8000 |