7VW5

Crystal structures of alphavirus nonstructural protein 4 (nsP4) reveal an intrinsically dynamic RNA-dependent RNA polymerase fold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.946
Spacegroup name	P 1
Unit cell lengths	67.450, 68.940, 71.420
Unit cell angles	116.31, 107.05, 95.10

Refinement procedure

Resolution	36.270 - 2.300
R-factor	0.1978
Rwork	0.197
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7f0s
RMSD bond length	0.002
RMSD bond angle	0.437
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.690	45.690	2.030
High resolution limit [Å]	1.910	5.700	1.910
Rmerge	0.074	0.026	1.658
Rmeas	0.105	0.037	2.344
Total number of observations	263495
Number of reflections	144300	5616	21199
<I/σ(I)>	6.21	23.02	0.56
Completeness [%]	87.5	89.5	79.7
Redundancy	1.826	1.894	1.741
CC(1/2)	0.995	0.998	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	18-20% PEG3350, 0.2 M magnesium acetate