7VUX
Complex structure of PD1 and 609A-Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-05-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.290, 68.410, 77.310 |
Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
Resolution | 48.910 - 1.640 |
R-factor | 0.1617 |
Rwork | 0.160 |
R-free | 0.20190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wt9 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.784 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.910 | 1.670 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.049 | 0.457 |
Rmeas | 0.067 | 0.618 |
Rpim | 0.045 | 0.413 |
Number of reflections | 70749 | 3505 |
<I/σ(I)> | 12.5 | 2.2 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.3 | 3.4 |
CC(1/2) | 0.996 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M Ammonium formate, pH 6.6, 20% w/v PEG 3350 |