7VPQ
Structures of a deltacoronavirus spike protein bound to porcine and human receptors indicate the risk of virus adaptation to humans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979150 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 201.225, 347.690, 255.114 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.360 - 3.100 |
R-factor | 0.2601 |
Rwork | 0.259 |
R-free | 0.27840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6atk |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.290 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 160248 | 25301 |
<I/σ(I)> | 5.28 | |
Completeness [%] | 99.4 | |
Redundancy | 6.1 | |
CC(1/2) | 0.964 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | solution containing 25% w/v Polyethylene glycol 1500, 0.1 M BIS-TRIS propane pH9.0, 0.1 M Sodium chloride |