7VPP

Structures of a deltacoronavirus spike protein bound to porcine and human receptors indicate the risk of virus adaptation to humans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-20
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.979183
Spacegroup name	P 41 21 2
Unit cell lengths	186.643, 186.643, 173.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.650 - 2.690
R-factor	0.1996
Rwork	0.198
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6atk
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.690	2.690
Number of reflections	84784	12941
<I/σ(I)>	10.69
Completeness [%]	99.2
Redundancy	10.68
CC(1/2)	0.997	0.472

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	solution containing 8% w/v Polyethylene glycol 1000, 8% w/v Polyethylene glycol 8000 and 20% w/v Glycerol