7VOX

The crystal structure of human forkhead box protein A in complex with DNA 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	C 2 2 2
Unit cell lengths	138.547, 144.291, 75.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.984 - 2.100
R-factor	0.1871
Rwork	0.186
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cby
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.107	0.062	0.616
Rmeas	0.113	0.066	0.653
Rpim	0.037	0.021	0.214
Total number of observations	406203
Number of reflections	44236	2372	2179
<I/σ(I)>	6.3
Completeness [%]	100.0	99.7	100
Redundancy	9.2	9.5	9.1
CC(1/2)		0.997	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES pH 6.0, 6% Tacsimate pH 6.0, and 20% PEG 4000