7VKE

Crystal structure of human CD38 ECD in complex with UniDab(TM) F11A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 32 2 1
Unit cell lengths	64.820, 64.820, 201.316
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.075 - 1.900
Rwork	0.193
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yh3 6pzw
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	1.830
High resolution limit [Å]	1.790	1.790
Rmerge	0.083	1.379
Number of reflections	47342	2686
<I/σ(I)>	12.1
Completeness [%]	100.0
Redundancy	10.1
CC(1/2)	0.999	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium Citrate, PEG 8000