7VHZ

Crystal structure of EP300 HAT domain in complex with compound 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-08
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.100000
Spacegroup name	P 1
Unit cell lengths	43.676, 89.219, 91.132
Unit cell angles	114.99, 95.33, 92.02

Refinement procedure

Resolution	19.980 - 2.000
R-factor	0.1924
Rwork	0.191
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bhw
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.980	19.980	2.120
High resolution limit [Å]	2.000	5.960	2.000
Rmerge	0.044	0.021	0.577
Rmeas	0.062	0.030	0.815
Total number of observations	144642
Number of reflections	78731	2736	12456
<I/σ(I)>	10.72	34.69	1.23
Completeness [%]	94.2	86.4	92.3
Redundancy	1.837	1.819	1.779
CC(1/2)	0.998	0.999	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	16% PEG3350, 0.1 M HEPES (pH 7.0)