7V6G
Structure of Candida albicans Fructose-1,6-bisphosphate aldolase mutation C157S with CN39
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.182, 56.536, 82.034 |
Unit cell angles | 90.00, 96.07, 90.00 |
Refinement procedure
Resolution | 39.821 - 2.343 |
R-factor | 0.2065 |
Rwork | 0.204 |
R-free | 0.25810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lnk |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.821 | 2.427 |
High resolution limit [Å] | 2.343 | 2.343 |
Number of reflections | 30829 | 3029 |
<I/σ(I)> | 12.29 | |
Completeness [%] | 99.4 | |
Redundancy | 6.7 | |
CC(1/2) | 0.997 | 0.871 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM Hepes pH =7.5, 11% (w/v) PEG 3350 |