7V6G

Structure of Candida albicans Fructose-1,6-bisphosphate aldolase mutation C157S with CN39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	160.182, 56.536, 82.034
Unit cell angles	90.00, 96.07, 90.00

Refinement procedure

Resolution	39.821 - 2.343
R-factor	0.2065
Rwork	0.204
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lnk
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.821	2.427
High resolution limit [Å]	2.343	2.343
Number of reflections	30829	3029
<I/σ(I)>	12.29
Completeness [%]	99.4
Redundancy	6.7
CC(1/2)	0.997	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM Hepes pH =7.5, 11% (w/v) PEG 3350