7V62

Crystal structure of human OSBP ORD in complex with cholesterol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.9
Spacegroup name	P 43 21 2
Unit cell lengths	111.880, 111.880, 367.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.580 - 3.250
R-factor	0.2146
Rwork	0.213
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zm8
RMSD bond length	0.003
RMSD bond angle	0.714
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	3.340
High resolution limit [Å]	3.250	3.250
Number of reflections	37700	6043
<I/σ(I)>	14.99
Completeness [%]	99.7
Redundancy	28.4
CC(1/2)	0.998	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	citrate, PEG4000, sodium acetate, GSH/GSSG