7V5N
Crystal structure of Fab fragment of bevacizumab bound to DNA aptamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.543, 130.599, 90.244 |
| Unit cell angles | 90.00, 103.59, 90.00 |
Refinement procedure
| Resolution | 19.710 - 1.700 |
| R-factor | 0.17952 |
| Rwork | 0.178 |
| R-free | 0.21002 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.048 | 0.511 |
| Number of reflections | 104111 | 15208 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | Sulfate |
