7V38
Crystal structure of NP exonuclease-PCMPS complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99311 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.227, 74.012, 140.807 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.793 - 2.403 |
R-factor | 0.2167 |
Rwork | 0.213 |
R-free | 0.26220 |
Structure solution method | SAD |
Starting model (for MR) | 4g9z |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.160 | 2.400 |
Rmerge | 0.085 | 0.049 | 0.496 |
Rmeas | 0.089 | 0.052 | 0.520 |
Rpim | 0.025 | 0.015 | 0.152 |
Total number of observations | 245203 | ||
Number of reflections | 20856 | 2266 | 1904 |
<I/σ(I)> | 10.8 | ||
Completeness [%] | 98.9 | 99.5 | 92.5 |
Redundancy | 11.8 | 11.3 | 10.2 |
CC(1/2) | 0.999 | 0.946 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Sodium malonate pH 6.0, 20% w/v Polyethylene glycol 3,350, 2 mM Sodium p-chloromercuriphenylsulphonate |