7V04
The N-terminal domain of PA endonuclease from the influenza H1N1 viral polymerase in complex with 6-bromo-3-hydroxy-4-oxo-1,4-dihydropyridine-2-carboxamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 75.421, 75.421, 119.317 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.320 - 1.910 |
R-factor | 0.2009 |
Rwork | 0.197 |
R-free | 0.23480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e6v |
RMSD bond length | 0.008 |
RMSD bond angle | 1.014 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.320 | 1.980 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.057 | 1.420 |
Rmeas | 0.058 | 1.455 |
Rpim | 0.012 | 0.314 |
Number of reflections | 29447 | 1580 |
<I/σ(I)> | 29.6 | 2.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 24.4 | |
CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.35 | 293 | 34% PEG 4000, 100 mM Tris (pH 8.35), and 220 mM sodium acetate |