7UXD
Crystal structure of APOBEC3G Catalytic domain complex with ssDNA containing 2'-deoxy Zebularine.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.489, 47.121, 52.042 |
Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
Resolution | 34.506 - 1.500 |
R-factor | 0.1736 |
Rwork | 0.172 |
R-free | 0.19440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6BUX (Chain A) |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
Rmerge | 0.086 | 0.079 | 0.468 |
Rmeas | 0.104 | 0.095 | 0.561 |
Rpim | 0.058 | 0.053 | 0.306 |
Total number of observations | 106388 | ||
Number of reflections | 33649 | 3094 | 3566 |
<I/σ(I)> | 7.7 | ||
Completeness [%] | 93.4 | 83.5 | 98.9 |
Redundancy | 3.2 | 3.1 | 3.2 |
CC(1/2) | 0.967 | 0.752 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG6000, 50 mM di-Sodium L-Malate; pH5.0, 30 mM CACL2 |