7USZ

Human DDAH-1, holo (Zn-bound) form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	44.968, 47.634, 146.965
Unit cell angles	90.00, 94.61, 90.00

Refinement procedure

Resolution	29.550 - 1.650
R-factor	0.1532
Rwork	0.151
R-free	0.19940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jaj
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.900	1.680
High resolution limit [Å]	1.650	1.650
Rmeas	0.094
Rpim	0.036	0.531
Number of reflections	73638	2000
<I/σ(I)>	11.2	1.8
Completeness [%]	98.3
Redundancy	6.8
CC(1/2)	0.998	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	100 mM Tris-HCl (pH 8.05), 21% PEG6000