7URU

Crystal structure of the low affinity Fc gamma receptor IIIA variant in complex with the Fc of IgG1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	72.983, 100.750, 124.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.430 - 2.400
R-factor	0.2176
Rwork	0.215
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sgj
RMSD bond length	0.012
RMSD bond angle	1.309
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.186	0.961
Rpim	0.077	0.655
Number of reflections	35352	1636
<I/σ(I)>	26.6	0.8
Completeness [%]	96.0	90.6
Redundancy	5.5	4
CC(1/2)	0.941	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	8% PEG 8000 0.1 M HEPES pH 7.0