7UNY
Crystal structure of D2 nanobody in complex with PfCSS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 182.416, 182.416, 143.728 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.490 - 4.130 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6waq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.497 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.490 | 4.460 |
| High resolution limit [Å] | 4.130 | 4.130 |
| Rpim | 0.071 | 0.485 |
| Number of reflections | 21543 | 4391 |
| <I/σ(I)> | 7.7 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.3 | |
| CC(1/2) | 0.989 | 0.615 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.6 M ammonium sulfate, 0.1 M sodium chloride, 0.1 M sodium HEPES pH 7.5 |
