7ULJ

Hsp90b N-terminal domain in complex with 42C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 65
Unit cell lengths	129.506, 129.506, 104.454
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.391 - 1.820
R-factor	0.1947
Rwork	0.193
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ucj
RMSD bond length	0.005
RMSD bond angle	0.838
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.391	42.391	1.890
High resolution limit [Å]	1.820	9.800	1.820
Rmerge	0.128	0.020	1.940
Rmeas	0.079	0.021	1.817
Rpim	0.028	0.008	1.158
Number of reflections	88988	586	8854
<I/σ(I)>	10.83
Completeness [%]	100.0	98.9	99.95
Redundancy	7.7	7.4	5.5
CC(1/2)	1.000	1.000	0.317

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M Sodium cacodylate pH 6.5, 0.1 M Sodium acetate, 5% MPD and 25% PEG8000