7UL0

Crystal structure of SARS-CoV-2 RBD in complex with the ridge-binding nAb EH8 isolated from a nonvaccinated pediatric patient

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-10-14
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.979
Spacegroup name	P 21 2 21
Unit cell lengths	81.771, 100.709, 106.019
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.910 - 2.490
R-factor	0.2266
Rwork	0.225
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s4s 7nab
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.490	5.380	2.490
Rmerge	0.144	0.084	1.037
Rmeas	0.148	0.086	1.070
Rpim	0.034	0.020	0.259
Number of reflections	31070	3254	3014
<I/σ(I)>	5.3
Completeness [%]	99.9	99.7	100
Redundancy	18.8	17.9	16.6
CC(1/2)		0.998	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	0.2 M sodium chloride, 0.1 M Na HEPES pH 7.5, 12% w/v PEG 8000