7UKV

Wild type EGFR in complex with Lazertinib (YH25448)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-15
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	I 2 3
Unit cell lengths	145.700, 145.700, 145.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.480 - 2.400
R-factor	0.2128
Rwork	0.211
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vh4
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.480	59.480	2.460
High resolution limit [Å]	2.400	10.740	2.400
Rmerge	0.090	0.032	1.572
Rmeas	0.100	0.036	1.757
Rpim	0.043	0.015	0.758
Total number of observations	103918	1220	7258
Number of reflections	19853	242	1468
<I/σ(I)>	10.8	34	1
Completeness [%]	98.1	93.7	97.7
Redundancy	5.2	5	4.9
CC(1/2)	0.998	0.998	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.0 M Sodium Citrate, 0.1 M MES pH 6.0