7UJV

Structure of PHD2 in complex with HIF2a-CODD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97933
Spacegroup name	P 21 21 2
Unit cell lengths	129.078, 37.597, 42.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.183 - 1.800
Rwork	0.173
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l9b
RMSD bond length	0.006
RMSD bond angle	1.368
Data reduction software	DIALS (2.1)
Data scaling software	DIALS (2.1)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.080	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.117	0.790
Rmeas	0.126	0.860
Rpim	0.047	0.334
Number of reflections	19834	967
<I/σ(I)>	8.4	0.8
Completeness [%]	100.0	100
Redundancy	7.1	6.5
CC(1/2)	0.998	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	32.5% (w/v) PEG 2000 MME