7UAI
Meprin alpha helix in complex with fetuin-B
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.8 |
| Number of Particle | 519760 |
| Software | crYOLO,ISOLDE,Coot,cryoSPARC,RELION,EPU,PHENIX,CTFFIND,Warp,UCSF ChimeraX,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
