7TZG

Structure of human LAG3 in complex with antibody single-chain variable fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	78.436, 142.928, 158.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.620 - 3.710
R-factor	0.2287
Rwork	0.226
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsi
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.620	45.620	3.810
High resolution limit [Å]	3.710	16.600	3.710
Rmerge	0.195	0.042	1.156
Rmeas	0.218	0.048	1.298
Total number of observations	98804
Number of reflections	19405	247	1394
<I/σ(I)>	7.43	24.21	1.65
Completeness [%]	99.8	94.3	99.5
Redundancy	5.092	4.166	4.961
CC(1/2)	0.994	0.999	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1% Tacsimate at pH 5.0, 0.05 M sodium citrate tribasic dihydrate, pH 5.6 and 7% PEG3350