7TXH

Human MRas Q71R in complex with human Shoc2 LRR domain M173I and human PP1Ca

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-17
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	72.959, 115.755, 137.804
Unit cell angles	90.00, 100.31, 90.00

Refinement procedure

Resolution	42.260 - 1.950
R-factor	0.1693
Rwork	0.167
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zk6 1x1s
RMSD bond length	0.008
RMSD bond angle	0.899
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.033	1.958
High resolution limit [Å]	1.950	1.951
Rmerge	0.077
Rmeas	0.088
Number of reflections	163294	1654
<I/σ(I)>	10.49
Completeness [%]	99.9	100
Redundancy	2	4.4
CC(1/2)		0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M NaH2PO4 pH 6.5, 12% PEG 8000