7TVA

Stat5a Core in complex with AK2292

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1271
Spacegroup name	H 3
Unit cell lengths	232.476, 232.476, 112.473
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.510 - 2.835
R-factor	0.2047
Rwork	0.203
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Previously solved structure in lab
RMSD bond length	0.006
RMSD bond angle	0.860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (3-FEB-2022))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.834	7.730	2.850
Rmerge	0.077	0.042	1.246
Rmeas	0.085	0.047	1.390
Rpim	0.037	0.020	0.612
Number of reflections	53485	2668	2669
<I/σ(I)>	9.1
Completeness [%]	99.7	99.4	100
Redundancy	5.1	5.5	5.1
CC(1/2)		0.996	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1.0M Na Malonate, 20% peg 400, 1% Jeffamine