7TTZ
Heterodimeric IgA Fc in complex with Staphylococcus aureus protein SSL7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 135.505, 165.447, 46.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.560 - 2.350 |
R-factor | 0.2025 |
Rwork | 0.200 |
R-free | 0.24750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qej |
RMSD bond length | 0.008 |
RMSD bond angle | 1.014 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.560 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.120 | 1.508 |
Number of reflections | 48763 | 3864 |
<I/σ(I)> | 14.8 | 2.15 |
Completeness [%] | 98.6 | |
Redundancy | 2 | |
CC(1/2) | 1.000 | 0.639 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% PEG MME 550, 0.1 M HEPES pH 7.5, 0.2 Magnesium chloride |