7TPB

p120RasGAP SH3 domain in complex with DLC1 RhoGAP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-11
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97918
Spacegroup name	H 3
Unit cell lengths	143.745, 143.745, 152.776
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.250 - 3.200
R-factor	0.2149
Rwork	0.212
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kuq 2j05
Data reduction software	HKL-2000 (2.3.8)
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.200	6.890	3.200
Rmerge	0.256	0.122	1.700
Rmeas	0.272	0.130	1.824
Rpim	0.090	0.042	0.657
Total number of observations	179535
Number of reflections	19702	1966	1952
<I/σ(I)>	5.3
Completeness [%]	100.0	99.8	100
Redundancy	9.1	9.2	7.8
CC(1/2)		0.992	0.332

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	0.2 M Magnesium nitrate, 16.5% PEG 3350