7TE7

Estrogen Receptor Alpha Ligand Binding Domain in Complex with RAD1901

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2021-12-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 65 2 2
Unit cell lengths	58.183, 58.183, 277.244
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.360 - 1.850
R-factor	0.22
Rwork	0.219
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ufx
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	27286	1330
<I/σ(I)>	10445
Completeness [%]	100.0
Redundancy	16.8
CC(1/2)	0.990	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3,350, HEPES pH 8, MgCl2