7T8W

Structure of antibody 3G12 bound to Respiratory Syncytial Virus G central conserved domain mutant S177Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	139.391, 139.391, 98.045
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.110 - 3.100
R-factor	0.2382
Rwork	0.237
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uvo
RMSD bond length	0.014
RMSD bond angle	2.026
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.050	3.310
High resolution limit [Å]	3.100	3.100
Rpim	0.164
Number of reflections	20300	3613
<I/σ(I)>	5.8	1.8
Completeness [%]	100.0	100
Redundancy	19.1
CC(1/2)	0.974	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	295.15	1.8 M ammonium sulfate, 100 mM sodium acetate trihydrate pH 4.4