7T2L
Crystal Structure of TEAD2 in a covalent complex with TED-662
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2020-09-24 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.651, 61.746, 79.753 |
Unit cell angles | 90.00, 117.33, 90.00 |
Refinement procedure
Resolution | 53.610 - 2.150 |
R-factor | 0.2058 |
Rwork | 0.203 |
R-free | 0.25660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e5g |
RMSD bond length | 0.006 |
RMSD bond angle | 0.798 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.610 | 2.490 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmeas | 0.107 | 1.088 |
Rpim | 0.055 | 0.579 |
Number of reflections | 14087 | 704 |
<I/σ(I)> | 10.6 | 1.3 |
Completeness [%] | 87.7 | 62.5 |
Redundancy | 3.7 | 3.3 |
CC(1/2) | 0.997 | 0.485 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium Formate 1.8M - 2.4M HEPES, pH 7.2-7.4 |