7SZC

Crystal Structure Analysis of human PRPK complex with a compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	73.290, 48.240, 128.550
Unit cell angles	90.00, 96.05, 90.00

Refinement procedure

Resolution	63.920 - 1.710
R-factor	0.1786
Rwork	0.177
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3enp 4ww5
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.920	63.960	1.740
High resolution limit [Å]	1.710	4.640	1.710
Rmeas	0.046	0.021	1.209
Rpim	0.024	0.012	0.633
Total number of observations	330553	16034	16957
Number of reflections	96583	4976	4810
<I/σ(I)>	15.7	51.6	1.2
Completeness [%]	99.4	97.9	99.9
Redundancy	3.4	3.2	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG3350, 200mM Potassium sodium tartrate