7SXJ

BIO-2895 (BRD0705) bound GSK3beta-axin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-04-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	68.090, 73.870, 82.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.940 - 1.850
R-factor	0.1849
Rwork	0.184
R-free	0.20340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kpl
RMSD bond length	0.004
RMSD bond angle	0.747
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.940	1.910
High resolution limit [Å]	1.850	1.850
Number of reflections	36269	3551
<I/σ(I)>	29.13
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M calcium acetate, 0.1M BisTRIS pH 7.0, 5% Glycerol and 17% PEG3350