7ST8
Crystal structure of 7H2.2 Fab in complex with SAS1B C-terminal region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-18 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.355, 43.030, 106.227 |
Unit cell angles | 90.00, 123.89, 90.00 |
Refinement procedure
Resolution | 26.410 - 2.750 |
R-factor | 0.2328 |
Rwork | 0.231 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3U9U Fab heavy chain and 2XQY Fab light chain |
RMSD bond length | 0.003 |
RMSD bond angle | 1.294 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.850 |
High resolution limit [Å] | 2.750 | 5.920 | 2.750 |
Rmerge | 0.161 | 0.069 | 0.503 |
Rmeas | 0.169 | 0.071 | 0.564 |
Rpim | 0.049 | 0.019 | 0.246 |
Total number of observations | 129654 | ||
Number of reflections | 13315 | 1430 | 1285 |
<I/σ(I)> | 16.9 | ||
Completeness [%] | 96.9 | 98.8 | 94.9 |
Redundancy | 9.7 | 14.5 | 4.6 |
CC(1/2) | 0.998 | 0.872 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M CHES, pH 9.5, 20 % (w/v) PEG 8000 |