7ST5
Structure of Fab CC-95251 in complex with SIRP-alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-11 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.0782 |
Spacegroup name | P 1 |
Unit cell lengths | 42.957, 97.704, 103.385 |
Unit cell angles | 77.18, 85.92, 85.74 |
Refinement procedure
Resolution | 50.010 - 2.200 |
R-factor | 0.2463 |
Rwork | 0.245 |
R-free | 0.27970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jjs |
RMSD bond length | 0.016 |
RMSD bond angle | 1.764 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.000 | 2.220 |
High resolution limit [Å] | 2.100 | 2.110 |
Number of reflections | 83446 | 12639 |
<I/σ(I)> | 6.3 | 2.3 |
Completeness [%] | 88.6 | |
Redundancy | 2.2 | |
CC(1/2) | 0.996 | 0.804 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES pH 6.5, 25 %w/v PEG MME 550, 0.01 M Zinc sulfate |