7ST3
Consequences of HLA single chain trimer mutations on peptide presentation and binding affinity
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2021-08-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 117.796, 118.041, 273.507 |
| Unit cell angles | 102.45, 102.45, 90.00 |
Refinement procedure
| Resolution | 50.480 - 2.780 |
| R-factor | 0.2395 |
| Rwork | 0.238 |
| R-free | 0.27470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7sr5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.577 |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.480 | 2.880 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Number of reflections | 340982 | 29320 |
| <I/σ(I)> | 9.76 | |
| Completeness [%] | 98.2 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.998 | 0.916 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100 mM Tris, pH 7.5, 200 mM Li2SO4, 1.6 M AmSO4 |
