7SSC
TRL345 lineage ancestor I8 Fab bound to an HCMV gB-derived peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-18 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.879, 74.669, 104.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 66.880 | 66.880 | 1.840 |
High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
Rmerge | 0.064 | 0.036 | 0.139 |
Rmeas | 0.070 | 0.039 | 0.153 |
Rpim | 0.028 | 0.016 | 0.062 |
Total number of observations | 287280 | 2766 | 17001 |
Number of reflections | 48200 | 469 | 2826 |
<I/σ(I)> | 17.2 | 23.5 | 9.6 |
Completeness [%] | 97.9 | 99.1 | 98 |
Redundancy | 6 | 5.9 | 6 |
CC(1/2) | 0.997 | 0.998 | 0.982 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M magnesium chloride hexahydrate, 25% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.5 |