7SR9
Human alpha-thrombin with 180- and 220- loops replaced with homologous loops from protein C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU R-AXIS IV |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-11-08 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.969, 78.525, 49.602 |
Unit cell angles | 90.00, 103.22, 90.00 |
Refinement procedure
Resolution | 30.460 - 2.100 |
R-factor | 0.1806 |
Rwork | 0.178 |
R-free | 0.22741 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ppb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.393 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL2Map |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 21066 | 21066 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 96.9 | |
Redundancy | 3.02 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate, 30% PEG4000 |