7SOX
Cryo-electron tomography structure of membrane-bound EHD4 complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 7.6 |
| Software | Dynamo,IMOD,PHENIX,SerialEM,CTFFIND,VMD |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING ONLY |
