7SO5
Novel structural insights for a pair of monoclonal antibodies recognizing non-overlapping epitopes of the glucosyltransferase domain of Clostridium difficile toxin B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-20 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.978720 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.167, 110.722, 83.060 |
Unit cell angles | 90.00, 99.33, 90.00 |
Refinement procedure
Resolution | 52.867 - 1.797 |
R-factor | 0.1869 |
Rwork | 0.185 |
R-free | 0.22290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bvl |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.880 | 1.890 |
High resolution limit [Å] | 1.790 | 1.790 |
Number of reflections | 95088 | 14702 |
<I/σ(I)> | 14.9 | |
Completeness [%] | 93.9 | |
Redundancy | 3.7 | |
CC(1/2) | 0.998 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M TRIS pH 8.2 and 39% polyethylene glycol 400 |