7SJP
anti-HtrA1 Fab15H6.v4 bound to HtrA1-LoopA peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2018-01-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.193, 90.212, 106.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.450 - 2.100 |
R-factor | 0.1698 |
Rwork | 0.168 |
R-free | 0.19880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7sjm |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.450 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.107 | 0.814 |
Rmeas | 0.117 | |
Rpim | 0.062 | 0.547 |
Number of reflections | 34460 | 2691 |
<I/σ(I)> | 12.7 | 1.8 |
Completeness [%] | 99.7 | 96.4 |
Redundancy | 6.4 | 4.4 |
CC(1/2) | 0.998 | 0.727 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 12% PEG 4000, 10% Ethanol, 5% glycerol |