7SI0
IgE-Fc in complex with 813
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.194990 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.958, 132.005, 177.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.590 - 3.000 |
| R-factor | 0.2128 |
| Rwork | 0.210 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hys |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.728 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.590 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 47037 | 4623 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.996 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M CAPS pH 10.5, 40%(v/v) MPD |
