7SHZ

IgE-Fc in complex with HAE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979460
Spacegroup name	I 1 2 1
Unit cell lengths	93.340, 187.340, 148.834
Unit cell angles	90.00, 103.13, 90.00

Refinement procedure

Resolution	47.350 - 3.000
R-factor	0.1882
Rwork	0.186
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hys
RMSD bond length	0.007
RMSD bond angle	0.954
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.353	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	48998	4592
<I/σ(I)>	12.94
Completeness [%]	98.2
Redundancy	3.4
CC(1/2)	0.998	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Sodium acetate pH 4.6, 8% (w/v) PEG 4000