7SD5

Crystallographic structure of neutralizing antibody 10-40 in complex with SARS-CoV-2 spike receptor binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	75.756, 79.845, 118.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.440 - 1.530
R-factor	0.17773
Rwork	0.176
R-free	0.20625
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7l5b
RMSD bond length	0.013
RMSD bond angle	1.783
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.440	1.530
High resolution limit [Å]	1.530	1.530
Rmerge	0.045
Number of reflections	111889	22517
<I/σ(I)>	17.5
Completeness [%]	97.1
Redundancy	7.4
CC(1/2)	1.000	0.256

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	310	0.2 M potassium thiocyanate, 0.1 M sodium acetate, pH 5.5, 8% PEG550 MME, 8% PEG20000