7S7C
Human Nuclear Exosome Targeting (NEXT) complex bound to RNA (substrate 2)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k),GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.62 |
| Software | RELION,Leginon,SerialEM,Gctf |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
